| Intonation settings assume the string is vibrating evenly across its whole lenght, and that the nut and frets are in the right places. So, if your strings are old, physically worn, have a lot of dead skin cells, grease, and other finger funk in the windings, or are otherwise worn out, change them.
Then make sure the nut's right. My experinece with Laklands (US and Korean) is that the nuts are done right (not a common feature on most production instruments BTW), but if the nut is too high, then it's going to play out of tune. A related issue is the neck's relief. If the neck's got too much relief in it, your intonation settingst that worked when the neck was more straight won't work now.
Those are the key factors on why it would have played in tune three months ago, but doesn't now- worn strings, and truss rod adjustment. Given the time (three months ago was early spring and now summer's just started. There's a lot of changes in the bass' environment over the change from winter through spring to summer so I would be suprised if the TR does NOT need adjustment.
I'd do this0 Buy a copy of Dan Erlewine's complete guitar repair book. Get Dan's book because he discusses the trade-offs involved in set up work (there's not one "right way" to do set ups), and he explains things in a very relaxed, player oriented manner. Learn to do your own set ups because it's a lot less expensive than paying someone to set intonation and tweak a truss rod. Plus you'll be able to really dial in what you want YOUR bass to feel like. And you'll learn an awful lot about what makes a great bass great and why there's so many just mediocre basses out there.
But if you got a gig and need to get that thing set righ now...
A. Tune all the strings to the pitch you're going to use.
B. Hold the bass in playing position.
C. Play the 12th fret harmonic and compare that to the open string. If they're not exaclty an octave, then you need new strings.
D. If the 12th fret harmonic and the open string ARE exactly one octave apart, then play the 12th fret harmonic and then play the note fretted at the 12th fret. The harmonic is your reference pitch, and the idea is to get the fretted note to match that reference.
D. 1. If your fretted note is sharp, that means the distance from the 12th fret to the bridge saddle is too short. Lengthen the string by moving the sadlle AWAY from the nut, towards the tail of the bass.
D. 2. If your fretted note is flat, the string is too long and you need to move the saddle towards the nut.
D. 3. Retune the string and repeat the process until you've got the two notes to match.
Then move to the next string until you're done.
jte
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JTE Spelling, grammar, and punctuation do matter, despite the threats of death by grease fire!
"Without space, music is just noise piling up on itself." TRK
Lakland Owners' Club # 248
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