| Sorry to say that some rooms are really not "fixable" by tone knob adjustments on the bass and/or amp. Or conversely, that in some rooms, it is impossible to get a good sound. I have unfortunately played many of those, often with different amps and also different basses, to no avail. I have tended to find this is true regardless of tone settings and volume, with the exception that the less loud the bass is, the less bad it sounds.
The sound that is excellent for things like organs and choruses and those environments which have the acoustics to make those sound so nice, are usually the same acoustical environments which are ill-suited to give you the direct punch, warmth, preciseness of an electric bass. The same is true for drums.
Another thing is that a room tested/soundchecked/demo-ed empty, particularly one that is inherently very live and "reverby" will react a little differently with a full service/attendance, but in general those reverby characteristics will remain, if muted a little by bodies.
In general, just realize that rooms with high ceilings and lots of hard surfaces (walls, ceilings, floors, etc and lots of echo when you clap your hands... make for challenging environments for electric bass. I wish there was a better solution, but I'm afraid you'll have to grin and bear the acoustics for this gig, and just know that you'll be playing again in your own church soon in a more sonically rewarding environment.
The best I could ever do was dial back the bass response, and dial back the highs/treble so that the mid-range (as nasally and awful hearing it soloed) could allow the bass to be heard without the mix of the whole ensemble turn to complete low-end mush with clacky treble note attack accompanying every pluck.
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Fretless Club Member #199/Fender Jazz Bass Club #78/Virginia Bassist #82/Earplug Club #1
Lawn furniture shouldn't have seatbelts.
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