DNA Testing Timeline

JAUQO III-X · #1 05-02-2011, 10:47 AM

PATC Articles- DNA Testing Timeline

Angus · #2 05-02-2011, 11:10 AM

It can be done SUBSTANTIALLY faster than that. Not the PCR step, though.

JAUQO III-X · #3 05-02-2011, 11:14 AM

Quote:

Originally Posted by Angus

It can be done SUBSTANTIALLY faster than that.

I know.

Muzoid · #4 05-02-2011, 11:17 AM

Dr. Phil does it in 30 minutes, or it's free - hahahaha

Musiclogic · #5 05-02-2011, 11:18 AM

After 6 paternity tests in my youth, I don't care about duration as much as accuracy.

CapnSev · #6 05-02-2011, 11:56 AM

Having formerly worked in forensics- yeah it takes a long time and its super expensive. I think I saw DNA evidence used like once or twice.

SoonerMatt · #7 05-02-2011, 12:05 PM

Having worked in various biology labs and done many DNA extraction and PCR experiments I can honestly say that if you know what you're doing you can isolate DNA, perform PCR, load it, run electrophoresis, and get results in 6 hours.

TheSuzie · #8 05-02-2011, 01:11 PM

Quote:

Originally Posted by SoonerMatt

Having worked in various biology labs and done many DNA extraction and PCR experiments I can honestly say that if you know what you're doing you can isolate DNA, perform PCR, load it, run electrophoresis, and get results in 6 hours.

Regular turnaround time for the certified microbiology lab I have used is 36 hours with single day turnaround available at an up charge. This is not for human samples nor is it for precise matching of an individual. This is for a standardized, quantitive, multi pass PCR used to identify levels of 36 different species of fungal mold. The test is performed to a high standard and the chain of custody, record keeping etc must be to a level that will stand up in court.

Last time I used them, I spoke to the tech in the mid to late morning and he said he had just got my samples and was preparing them. I had the results in my email by the end of business that day.

Selta · #9 05-02-2011, 01:16 PM

Quote:

Originally Posted by JAUQO III-X

I know.

So, what then exactly is your point here?

Hoover · #10 05-02-2011, 01:21 PM

Quote:

Originally Posted by Ray Salamon

So, what then exactly is your point here?

I LOL'd.

SoonerMatt · #11 05-02-2011, 01:21 PM

Quote:

Originally Posted by TheSuzie

Regular turnaround time for the certified microbiology lab I have used is 36 hours with single day turnaround available at an up charge. This is not for human samples nor is it for precise matching of an individual. This is for a standardized, quantitive, multi pass PCR used to identify levels of 36 different species of fungal mold. The test is performed to a high standard and the chain of custody, record keeping etc must be to a level that will stand up in court.

Last time I used them, I spoke to the tech in the mid to late morning and he said he had just got my samples and was preparing them. I had the results in my email by the end of business that day.

Admittedly I worked almost exclusively with Thalia dealbata but even still it was a 6-hour turnaround when using a kit. I assumed DNA isolation kits for blood, which I'd imagine are used much more frequently, would work just as quickly.

Schlyder · #12 05-02-2011, 01:24 PM

So how long would it take to positively identify a human to 99.99999% ??

Angus · #13 05-02-2011, 01:31 PM

Quote:

Originally Posted by Ray Salamon

So, what then exactly is your point here?

Yeah, exactly. This tends to be how his threads go.

Nomadskills · #14 05-02-2011, 01:36 PM

Quote:

Originally Posted by Hoover

I LOL'd.

+1

drerio · #15 05-02-2011, 01:37 PM

Quote:

Originally Posted by Schlyder

So how long would it take to positively identify a human to 99.99999% ??

Accuracy depends on the PCR enzyme. The old Taq enzyme would be suspect, but advances have made this accurate. Thermal cyclers can be run in less than half a day the restriction enzyme incubation in less than two hours. Gel run for specific markers in about 30 min. Then fragments will need to be run for GATC accuracy - depending on the equipment... About two hours. To ensure accuracy you would want at least three labs running this simultaneously to eliminate any human errors (pipetting, etc). I suspect the military had their own and probably contracted verification with labs close by (India, China, etc). Pretty basic procedure.

blendermassacre · #16 05-02-2011, 01:47 PM

Quote:

Originally Posted by Ray Salamon

So, what then exactly is your point here?

making a mess.

Musiclogic · #17 05-02-2011, 01:48 PM

Quote:

Originally Posted by Angus

Yeah, exactly. This tends to be how his threads go.

Jauqo is a curious sort, I think he is truly curious to see where people take things, and how different people approach things of a semi ambiguous nature.

I find it interesting personally, but who knows. Jauqo could have completely different reasoning. I'm just guessing...LOL

blendermassacre · #18 05-02-2011, 02:06 PM

Quote:

Originally Posted by Musiclogic

Jauqo is a curious sort, I think he is truly curious to see where people take things, and how different people approach things of a semi ambiguous nature.

I find it interesting personally, but who knows. Jauqo could have completely different reasoning. I'm just guessing...LOL

Especially from the thread last night, and this one as a follow up, it's sort of like a ****-mine field. He just sort of sets the trap and watches the aftermath.

TheSuzie · #19 05-02-2011, 02:08 PM

<--- dense. I never even thought what this may be about..

JAUQO III-X · #20 05-02-2011, 02:23 PM

I'm not here to make a mess at all.

People come into my threads and start a mess.

I was having a conversation with someone earlier about DNA testing and since a lot of people seem to be curious about some aspects of what DNA testing is about I thought I would post some info.