| +1. If it only happened on that one gig, chances are there was something about that room that was making that frequency louder.
Moving the amp/cab around may have helped. Was it in a corner, is your amp rear ported? Was it on a hollow wooden floor? Could have been any number of things. If it happens again at the same venue, enlist a couple of band mates to move your amp (very carefully), even elevate it, whilst you're playing to tune the offending frequency out and find the sweet spot.
I don't think a compressor would help, as it compresses all of the sound going into the amp. The room was reacting to the sound coming out of the amp, so it would have sounded just as loud on that note.
However, what would work would be a parametric EQ as it will affect only a portion of the sound coming out of the amp by honing in on the offending frequency and reducing it's volume. Luckily you know which note it is, so you should be able to find the corresponding frequency. Or just boost the gain on the eq, sweep the freq knob through whilst playing till it jump out at you, and then reduce till it sits better with the other notes. If you have a 'Q' or width control, you can really hone in tightly on the offending frequency without affecting the other notes. This is more or less the same process used to reduce feedback, and in mastering.
The eq really shouldn't be necessary though, but it's a viable solution if moving the amp isn't an option.
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