I'm blessed with good basses, a great set-up guy, and good ears - I was a professional trombonist many years ago. Predictably, as I play more bass, the fretless bug has hit. I know I should get with a teacher and internalize a left-hand fingering discipline, but I'm just having a ball right now, and have used the fretless on my last few gigs. So, the question. I recently got a Lakland prototype 34" 5-string, which is a bolt-on, as compared with the neckthrough Pedulla and Yamaha I have on hand. All axes were set up by an excellent tech, and I have the same strings on the Pedulla and Lakland. The Lakland ergonomics work a lot better for me - I got it back from set-up last night and was able to play for two hours without any wrist or hand weirdness, which I cannot do on the Pedulla. BUT it seems a whole lot harder to play in tune than the Pedulla or Yamaha - and while the Pedulla has lines, the Yamaha does not, and it does not present the same difficulty as the Lakland (which has side dots and lines, but no lines across the fretboard). In listening, it seems that the Lakland's notes have a lot more of the upper harmonics coming through, which I understand is typical for bolt-on as compared with neckthrough. But I'm wondering if this also means that intonation for each single note has to be dialed in that much more, so that the upper harmonics line up right for the specific key in which you're playing, whereas the fundamental-rich neck-through is "easier" to play in tune because you don't have the complication of the upper harmonics. It's almost like the neck-through resists the even-tempered scales implied by the fretlines on the Pedulla. OTOH, I've thought of those lines as a distraction. Am I on drugs? Anyone else have thoughts on this?