Well, I gone and done messed up my intonation trying to learn to adjust things myself. I read the sticky which points to some pages to read and printed those out & read them. I have a 2005 MIA Fender V and can't get the E string intonated for the life of me. I now have a few questions and need some TB wisdom. According to the stuff I've read, the 12th fret harmonic should be the same tuning as the fretted 12 fret. If the fretted note is sharp, you are supposed to lengthen the scale by adjusting the saddle away from the nut. I've been adjusting by 1/8 - 1/4 turns for over 1/4" back from the saddle on the A string and it still fluctuates between 12-20 cents sharp. Jeez, do I keep going?? The saddle is about at about 34-5/16" from the inside of the nut now, and almost even with the B string (which is intonated) saddle. Why can't I get this string intonated?? It is not a tapered string, and it's a .105 or .110 while the B is a .130. Anyone else experience this? Should I put the saddle back a little behind the one on the A string and start over? Any help greatly appreciated!