Good morning everyone, I am fairly new to doing my own set ups and I am trying to set up my Squier Jaguar Short scale bass. I was pretty proud of my self because I straighten the neck and got my action to a good level. I intonated each string but the E string is giving me problems. The first 4 frets are sharp(backwards on the tuner) and no matter what I do it doesn't help. I screwed the bridge forward almost all the way and backwards almost all the way and no difference. I can get it to a point where the 12th fret matches the E but no luck on 1-4 and few other frets. Any one have any suggestions? I realized that I was supposed to loosen the string before adjust the intonation but I haven't been. Will there be any consequences for that? When i adjust one string do the others need to be adjusted as well?