Does anyone know why the fifth fret (D) is significantly louder than the third fret (C) on my A string?
We need to know more: how about the E on the 7th fret, and these notes compared to the other strings?
Perhaps your bass resonates strongly with the D? Is the open D string just as loud as the D on the A string?
Might just be the note that is louder in the environment. I play at our church, and on any bass I play, E string 3rd fret(G) booms very loud, while all other notes are at regular volume. Octave up G does the same thing, just not as much volume difference. Seems that it's the note that resonates most in the building. Try playing octave up D, D string...12th fret, or G string...7th fret to see if it has the same volume difference.
That's also very true. My house sounds like its going to fall apart when I play the E an octave up from open yet the others sound a lot less intense
Try tuning the A to Ab and see if the loudness follows the note up one fret to the sixth. Then you'll know if its the room or the bass.