Okay ... I've got a recent problem with a very nice bass that I've had for a number of years. I've been noticing that even when I've just tuned, fretting a fourth, for example, on my D and G strings, is always out of tune. It gets worse as I go up the neck. I tried tuning various different notes up the neck after tuning the open strings. The G is consistent, if not slightly flat on a couple of notes. The D string, though, gets progressively more sharp as I move up the fretboard, to the extent that once I play a high "A" (19th fret), it's almost 50 cents sharp! I've never had this trouble before, and I'm hoping it's a setup issue. Peace.