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Double Bass A-string

When tuning a small 1/2 bass I have, I can tune all the string fine. I play G on the E string and it fine. F on the D string is fine. They are both in line with each other fret wise, but my C on the A string is not inline with the others, although the A string is int tune. I hope that makes sense. I am an over thinker therefore and over explainer!