Just an FYI - dropping the gain to 10 o'clock an running your master up to 3 o'clock makes the amp work harder and makes the signal noisier. You need to find the sweet spot on your gain with your bass. Unfortunately the Rumble amps don't have a set gain light or a gain clipping light, so you have to use your ears.
What you are trying to do is give the power section the strongest clean signal possible for it to amplify. If you send it too weak of a signal, it has to work too hard to bring it up to your expected level and the sound although not distorted, is not as clear as it can be. It doesn't have as high of a signal to noise ratio because running the power section hotter adds noise from the power section. Conversely if you run the gain to high, it delivers a distorted signal to the power section to amplify, and it is not a desirable distortion like your drive channel is. The sweet spot gives you the clearest, cleanest, and loudest sound from your amp. But since you don't have a gain set-light or gain clipping light, you're really going to have to use your ears to find it.
To find it, go ahead and start with your gain at about 9 o'clock and play a pattern that goes all the way from lows on the E string to highs on the G string. Be sure to include your loudest and lowest notes you'll normally play in a gig. Then turn it up to 10 o'clock and you'll already hear a difference. Continue playing the pattern and moving the gain up an hour as you go. You will make one of those moves, perhaps between noon and 1 o'clock, 2 o'clock and 3 o'clock, or wherever, and the sound really and suddenly comes alive. It is noticeably clearer and louder. That's when you've found the general area of your sweet spot. Now to refine that, move the gain control down 1/4 of an hour at a time until you hear that sound drop in both level and clarity - it will sound duller. Move it back up until you just hear that cleaner/clearer sound and WRITE IT DOWN! That's the sweet spot for that bass.
If you use a different bass, it may very well be in another spot, especially if you are switching from a passive bass to an active bass. With the battery-driven active electronics on an active bass, it typically sends a much hotter signal to the bass input, which is why most amps have a pad button - The Rumbles don't have that. A Pad button (or an "Active" input) will typically drop the signal from your bass down by 10db. Whereas an active bass in the normal input will send such a strong signal that you need to turn it down to 3 o'clock, you really don't get any granularity of adjustments from there. The tiniest adjustment will make a huge difference. So with a pad button used on a hot signal, it allows that gain-set sweet spot to be somewhere between about 10 o'clock and 2 o'clock with much more granularity of adjustment possible inside that range.
If you do use a second bass, either active or passive, find the sweet spot on the gain for that bass using your Rumble 100 and WRITE IT DOWN. A simple thing to do is write it on a note card and put the note card in your bass's case or gig bag. Be sure and include the brand and model of the bass the note card applies to so you don't have any confusion when you're gigging.
Also, besides the information I gave you above, be aware that you may just plain have too little rig for the gig, especially since it only puts out 100-watts and has just one 12" speaker. I would call your venue a large medium-sized room and would NOT expect that rig to handle it. Be aware that if you are running a DI out to a soundboard for FOH system, you're still going to need to be able to hear yourself. Setting that combo up on an amp stand or even a chair will help, but if you have a loud drummer and enthusiastic guitarists in the group, It will be rough to hear yourself. I've used 2x112 cab set ups for medium-sized venues just fine, but I was feeding them a LOT more power. Bad news is you can't add an extension cab to it.
Also, be aware of the distance from the stage to the back of the room. Long rooms eat your sound. I would expect a Rumble 500 to be good for that room, although without FOH reinforcement you would probably need a Rumble 210 extension cab so you end up with a 500-watt 410 solution. Another 100 occupancy would make it a large venue and 100 less would make it a "real" medium-sized venue (IMHO).
To give you some perspective on that, back in the early 70's I was touring for 3-years playing 5-nights a week, 50-weeks per year. I had a 1966 Fender Bassman blackface amp that I had bought new in 1966. It was a 50-watt rms, all-tube amp, and I'd used it through high school and some college with no problem at all being heard. Then about 2-months after I started touring with it in the Summer of 1971, our guitarist and our keys players both bought Vox Super Beatle amps (120-watts with 412 cabs). We were playing a large club (occupancy 900+) in Lincoln, Nebraska and they just buried me. Everybody was telling me to turn up. My amp couldn't handle it. If I turned it a hair over #7 on the dial it would fart out those standard Jensen speakers. Just not a worse feeling than when everyone tells you to turn up and you're already maxed out. Your situation sounds very similar to what I ran into. I don't think your Rumble 100 is up to the task.
Next day I went into a local music store and came back with an $1100 Standel piggy-back amp ($8,563.73 in 2024 USD). It was only 200-watts, which is not a lot now but was a LOT back then. That was the same power as the acoustic 360/361 which also had a 200-watt powered cab. The speakers were perfectly matched to the amp and I could turn it all the way up and hit my low E without getting any distortion out of it, while shaking everything off the walls. That night I found out I could not turn it up above #3 in that large club in Lincoln. I never again had "not enough rig for the gig".
Currently I can get that same level of sound at wide open Music Festivals using my Eden WT500/800 bridged at 800-watts into my 8Ω DNS-410 cab (handles 1400-watts rms). It's a lot more power and it sounds a lot better because those custom speakers are FAR better than anything available back in the 70's.
I have a number of rigs I use though depending on the size of the venues. For small venues I use a single Eden EX-112 driven by a 225-watt Eden TN226 class D amp. That's twice as much power as your Rumble 100 and I only use it for small venues. I have used two of them powered by a much bigger amp for medium-sized venues and that would also handle smaller Large-sized venues. I now use my 8Ω DNS-210 for medium and large venues driven by 400-watts from my DNA-800 amp.
If you decide to upgrade...don't go half way because you'll just need to sell that amp and buy a better one in a year or two. If you are going to stick with the Rumble series, go right to the Rumble 500. It is a 210 cab powered by a 500-watt class D amp that will put out 350-watts into its internal speakers and a total of 500-watts (250-watts each cab) when you add a Rumble 210 extension cab. That combo by itself is fine for small to medium-sized venues. Large and outdoor venues are going to need the additional speaker cab and that 500-watt x 410 rig will be enough "rig for the gig" just about anywhere. If you expect to continue gigging, I would highly recommend you get at least a 500-watt (better yet 800-watt) amp with either a 410 cab, or get 2x210 so you don't have to lug a 410 to venues where the 210 will do.