I will start by admitting that I am not a seasoned bass player - but I do record bass riffs and piece my compositions together in Cubase. I have 5 string Ibanez Ergodyne with active pickups. Something I have noticed today is that when I play the same 2 notes on my E-string, each note is not in tune with the other. Specifically, the second note is always higher. I have my index finger on the 7th fret I don't think I'm applying more pressure on the fret when I'm playing the second note and instead of alternate picking, I've even tried using the same finger to try and get the same attack and pressure for both notes. I went through about 10 takes and they're all doing the same thing. I know I can consciously vary my pressure on the second note to lower the note that's produces but I'm just curious to know your thoughts on this. Suppose I should grab a pick and see what happens too..