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I'm no expert on all the possible pedal and buffer/bypass combinations, but let me say that some pedals (like a tuner or other occasional use only effect) may only act as a buffer when the pedal/effect is engaged. For instance, the Polytune if I recall is true bybass and so theoretically it should act as if it wasn't even there if it is switched off. In contrast, I believe most Boss pedals in fact have a buffered bypass.Just make sure I'm clear on this- if a DI is placed after something that buffers the signal, such as a compressor/limiter or even a simple Polytune tuner, the signal essentially is "active" and buffered and one could theoretically use a passive DI with optimal results?