On the subject of Tone Effects From The Wooden Frame, yes, there is a big difference between bolt-on and neck-thru frames. Neck-thru construction limits what you can do with the body structure. In a neck-thru, that spine of neck wood down through the middle of the body is a strong structural reinforcement. That spine almost guarantees that the body will be very rigid and stiff, regardless of what you do with the construction of the wings.
When we're building basses to have a warm, rich sound down in the midrange, the main thing we do is to soften up the body. Softer woods, chambers, internal structural tricks, to weaken the body and make it flex more with the vibration of the strings.
If you are trying to build a bass with the emphasis on bright high end pinging and panging, then overall frame stiffness is your goal. Build the neck and body very stiff and bring on those screaming pickups. Neck-thru construction makes it easier to build it stiff, because of that strong spine running through the body.
If you are trying to build a bass with a warmer, richer tone, then bolt-on construction allows you to use softer woods and weaker structural design. The neck and body are two different structures and you can adjust the design of them separately.
Now, these aren't absolutes, because there are a lot of variables and other structural tricks. You can make a soft, warm neck-thru with some extra effort. Like seriously chopping away that spine to weaken it. And you certainly can make a very stiff body on a bolt-on, if that's what you want. Heavier wood, extra thick, internal bracing, maple back, etc.
But, in general, a bass with neck-thru construction will have a stiff frame and bright tingly sound. That may be what you want.
If you are looking for more mid-range richness and less tingle, you are probably better off building bolt-on or set-neck construction. That will give you more options in the construction of the body.
(I removed my grouchy old man comment)