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Just one string going out

Hi -
Had a bass stored for a few months. (in closet). (while I played another one).
Tomastik flat wound strings on a Jack Casady.
I notice that the E string does not want to stay in tune, while the others do.
Strings are a few years old. Does this mean it is time to change them? Not trying to
be dense - I notice that Carol Kaye says that she keeps hers on a really long time and Danny
Mo Morris has a set on his bass for over 15 years!
Any thoughts on why just one string is going out? Practical things to check?
thanks
 
Simple obvious question but are you always tuning "up"? I have a friend with a Musicmaster that was always going out of tune - E-string mostly. The machines on that bass are not especially nice and have a lot of backlash/slop.
His trouble was that he would tune passed E and then back off. It would hold for a little bit but then the slop in the machine would allow it to slip flat. Once he started only tuning up to the E and never down, the problem went away.
Sorry if that is too "tuning 101".
 
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