Yeah, I've messed with that but I get nothing out of it.
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I don't know what the layout is on your interface, but for me as long as I have my bass plugged into one of the mic preamps, with the "gain" set high enough for the signal light to go off when I play, the "mix" knob set fully counter-clockwise, and the "Level" knob turned up high enough to hear, I can always hear myself, with or without any software going.