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I have the holy grail for everybody, with this you can isolate every instrument or voice (not only guitars), also you can play the full song without an instrument, for example the bass.
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And here the video
Roland r-mix
Sony spectralayers
or
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Whenever it says "remove" remember that you can invert this to isolate what you removed. There's youtube videos on de-constructed songs that isolate each instrument.
the riffstation looks close but it says it isolates the guitar, not the bass, its weird they make devices like the Tascam bass trainer were you can remove bass to play along with songs , why not just the opposite, were you can remove everthing you want, maybe leave just the drums, or whatever.
Mixed mono or stereo tracks cant be unmixed.
Haven't tried Riffstation, but it sounds similar to Transcribe from http://www.seventhstring.com/ at $40 USD. It's been mentioned in various similar threads before.