First of all, they're screwed on, not bolted 
Yes, there is a difference.
Secondly, can you hear the difference?
A while back I was comparing two very similar basses & the thought never occurred to me, at the time, that part of their difference in timbre was due to the neck-through vs. screw-on designs.
Now I'm rethinking every time I tried a neck-through Ibanez BTB & wish I'd taken that into account.
I'm also wishing I could get a SR505E (screw-on) & BTB740 (neck-through), both with Bartolini BH2 pickups, side-by-side for a comparison.
Yes, I know there's been much discussion in the past, I'm reading those threads now.
However, I don't think there was a poll.

Yes, there is a difference.
Secondly, can you hear the difference?
A while back I was comparing two very similar basses & the thought never occurred to me, at the time, that part of their difference in timbre was due to the neck-through vs. screw-on designs.
Now I'm rethinking every time I tried a neck-through Ibanez BTB & wish I'd taken that into account.
I'm also wishing I could get a SR505E (screw-on) & BTB740 (neck-through), both with Bartolini BH2 pickups, side-by-side for a comparison.
Yes, I know there's been much discussion in the past, I'm reading those threads now.
However, I don't think there was a poll.