You want a tight & focused sound for every room you play in? #1. You have to make sure your playing techniques and the rig you have can create the sounds you desire. If you are playing near the neck all the time, it might serve you better to pluck in the middle and/or towards the bridge more. Your boomy tone will automatically get less boomy. If your speaker cab is not delivering the tone you want, you can get CLOSER to it with EQ, but you will probably always fight the cab in the end.
If your rig and playing techniques achieve the sounds you want, but you don’t know how to get the tone you want in different venues, invest in a separate EQ pedal. The Empress ParaEQ is a great choice. I have a GK 1001RB and the EQ on there is great for bass tones, but not correcting a tone at a show. The Empress pedal allows me to pinpoint a strong frequency and reduce it. The pedal allows me to roll some lows between 30hz and 150hz while simultaneously removing some of that muddy range between 200hz and 400hz. Each venue will present a new version of your tone. The key is to REMOVE the frequencies that are too strong to allow the other parts of your tone to come through. Don’t purely boost frequencies until you get what you want, it’s likely to not blend in well with other instruments and will result in you despising your bass tone throughout the show.
There is a bit more to this, but I wanted to give a somewhat view on it. These principles have been working for me in every venue I play and I believe they can help you too.