Vocals, acoustic guitar, and other instruments have a dominant hump around 250Hz. The chestiness of a male vocal, and the proximity effect of most stage mics, and even the drums, all have that hump. This builds up in the mix, and the cluster of 250Hz humps makes it a problem frequency. I did sound at a church (as well as playing bass) and we had a lot of musicians and singers. I started working on cleaning up the sound in the PA. First, I low cut everything but the bass and kick. Next I trimmed the lows out of each singer's mic. I shot for very natural sounding vocals, without any of the proximity bass boost everyone had been used to. Then I took out un-natural humps in each instrument, creating a sonic space for each instrument. At the time, I hated 250 in my bass tone, so I cut as much there as I could. I liked my bass tone below and above the other instruments, and they fit nicely in between.
It worked incredibly well. Three guitars (one acoustic, two electrics), flute, keyboards, drums, bass and 5-8 singers. (the flute player had her own pedalboard, with panning delay, octave below, and intelligent harmonizer) All was under control and no 250Hz hump anywhere.