You mentioned changing the power transformer. If it is closer to the power tubes, they may be picking up hum from the transformer. I don’t know if shielding or rotating the power transformer 90 degrees would help in this case.
If the tubes are not well matched, despite what the seller claims, badly balanced push and pull sides can result in hum. A simple test is to swap the positions of the power tubes and see if the noise changes. If you have an infrared temperature gun you can see if any of the tubes are running hotter than the others.
When running 6550’s in a vintage V4B, some add 1K 2W screen resistors pin-4 at each power tube R41, R42, R47, R48 to extend tube life. This comes with a little power loss.
I’d also check the 6.3V heater to ensure that the proper heater voltage is present with the 6550’s current draw.
A nice clean schematic:
Service manual.
Just to be clear, 6550’s should not be used in the current V4B reissue. It is optimized for 6L6GC tubes.