I always remove the casters from my Ampeg B100R and my 63 Portaflex. Some people will say it doesnt make a difference - but it does to me. Here's some info from the old Ampeg Portaflex manual that you might find interesting reading:
"As mentioned, models B-15N and B-12X are factory equipped with detachable, shock mounted dollys. IMPORTANT: Remove dolly while amp is in use. Since the dolly will absorb vibration, it will also absorb sound waves. There is a definite gain of power if the amp has a solid footing. You may seemingly get enough power with the amp on the dolly, but the speaker is working harder than it should be. You are in effect, spinning your wheels on the ice."
And this is from the Barefaced cabinets site regarding mechanical coupling of a cab with a stage or floor:
"With mechanical coupling the stage/floor is excited by the vibrations from the cabinet or by the acoustic output from the speakers/port and the floor itself becomes a resonating surface like a giant drum skin or speaker cone. The acoustic output from the resonating floor is very unpredictable, centred around the modes of resonance of the floor, which almost always results in a boomy onstage sound and unpredictable offstage sound (depending on how it reinforces and cancels the output from the speaker cabinet)."