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Obviously its important to use the proper size tools. It often makes it easier to loosen the strings first, but I rarely do unless it doesn't seem to be adjusting. For intonation I find I'm often turning the screw but the saddle can't move under the string tension. So you're really just unscrewing the screw. Loosen that string, move the saddle by hand, tighten the screw.
When the intonation screwhead is behind the bridge, some saddles won't move only if you are attempting to make it intonate sharper, then the screw can back away from the bridge. If you are sharp and trying to go flat the saddle has no choice but to move; that increases tension on the string though, and may cause damage to the screw. The solution is to have intonation flat and loosen the screw to sharp, but push on the screw with the tool to force the saddle forward. There is no need to loosen the string.
When the intonation screwhead is in front of the bridge, everything is reversed. The screw can back away when going flatter yada, yada, yada.
That is odd but not beyond the range of possibilities. When I intonate a string, when going sharper, I loosen the tension on that string so as not to damage the screw’s threads, then tune and check intonation again. Either the screw or saddle was defective (threads missing or damaged) or someone before it was sold stripped the threads.