Well actually the neck-through vs bolt-on debate has always been based on the premise that wood is better sounding when glued to wood, and that one single piece of wood must sound better than two bolted together. In fact this only works when the two pieces of wood are of the same species and contain a similar grain pattern, moisture content, and are genetically within 12 centimorgans of each other. That in turn assumes that there is a corresponding recombination frequency of 12%, and then the only discerning factor is the moisture content, which must be between 6% and 9%, unless the wood has been roasted and in that case it is between 3% and 5%. However, if the wood has been roasted and the genetic distance is similar then we must take into account the chemical composition of the glue used along with of course the grit of the sandpaper used to finish the wood, which generally starts at 150 grit and proceeds through 180, 240, 320, and potentially higher if the wood has an oil finish, or alternatively if the wood has a clear finish the then the finish may be sanded/polished through 12000 grit using a wet sander. This generally affects the tone in terms of "smoothness" of sound. Of course you can't leave out the nature of the screws/bolts (in the case of a bolt on) and whether the bolts are simple wood screws or machine screws that are connected through threaded inserts, and whether those threaded inserts are brass, stainless steel, or some other material. Then there may be the potential of galvanic corrosion between the screw/bolt and the threaded insert, which may cause a varying degree of alteration in the tone over time. This results in tone that is described as "sticky" or "hard" and may result in the use of curse words. If graphite rods are inserted in addition to the standard truss rod there is "less" wood to resonate and this can in turn be mitigated through the use of a "high mass bridge" which may interact with a single cut bass in ways that are different from double-cut basses. The country of origin most definitely affects the tone, as does the year of manufacture and whether a brass nut is involved in the manufacturing process. Finally strings are a huge factor, and "flat wound", "round wound", "pressure wound", or "un wound" strings can drastically affect tone and sustain.
