My band plays in drop C (CGCF) tuning with 2 guitar half stacks...The low bass peters out noticeably.
For starters, drop C is 32.7 Hz
My RTA analysis of both D410-XLT and Avatar Neo 410 show these drop like a rock at 32 Hz.
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The 1st harmonic of the 32 Hz fundamental is 64 Hz.
The charts both show 60 Hz as the starting point for severe rolloff.
If you are a player who hears only the 1st harmonic, you should hear no droop.
However, you hear noticeable droop, so the fundamental is important to your ear.
This requirement for 32 Hz mandates a subwoofer as a separate cab to your existing setup.
To get down this low you need either a very big box or a lot of power.
Bi-amping and active crossovers are required, so this is a conflict with your existing tube head.
You could probably split the input signal to a 2nd preamp and poweramp dedicated to the sub.
Very few heads are bi-amp friendly.
This is why bi-amping is best suited for separate pre and power amps.
The typical 4x10 averages 100 to 106 SPL, 1 watt at 1 meter.
Subs capable of 32 Hz have SPL of 88 to 94, 1w/1m.
At best, this is a 12dB spread, or 16x more amplifier needed for the sub.
You need a minimum of 16 watts sub for every 1 watt 4x10.
Two Eminence LAB15 require a total internal volume of 4.58 cubic feet to hit an F3 of 34 Hz.
These are 88 SPL each, or 94 SPL as a mutually coupled, parallel pair.
These are 6 ohm nominal drivers, so a parallel 3-ohm load is presented to your subwoofer amp.
My PLX-3002 would drive a pair of these to full power.
At a power ratio of 12:1, the 2xLAB15 at full 600w input power is matched by your tube amp at just under 38 watts.
At 12:1, the 250w tube head at max output requires a 4,000w sub amp which far exceeds the rating of the LAB15 pair.
The 2xLAB15 might be workable if you don't drive the tube head above 38 watts.
Venues do not have 4,000 watt circuits available on stage.
Nor do they have room for three 2x15 and multiple sub amps.
To get an efficient sub that matches your 250w tube amp and current cabs, you have to move to a bass horn.
A 36" wide T-48 will get you the depth and loudness required to keep up with the tube head.
Bi-amping is still required, but with a smaller power amp.
T48 in 36" hits 102 SPL, 40 Hz, 1w/1m.
You will need a trailer for T48.
I have one sitting in my 5x10 trailer, and three more ready for assembly in my garage.
The size of these (48" x 36" x 24") is not for the faint of heart.