I usually connect a signal generator to the power amp in jack so I can't tell you where the volume knob would be for 25V
RMS.
I can tell you that 35V
RMS is around 300W into 4 ohms. 25V
RMS into 4 ohms is about 160W. P= V
2/R so (25 V
RMS)
2/4 = 160 W. A low frequency sine wave into the amp with 25 V
RMS into a 4 ohm cab, putting out about 160W, is going to be very loud. That's why they use dummy loads.
Just to provide some background. You set the balance because you want the positive going part of the output signal feeding the power tubes to be the same as the negative going signal. When they are the same, the waveform is balanced. Here are a couple of pics. The sine wave on the top is asymmetrical or unbalanced; the one below is balanced. So the balance pot is adjusted to provide a signal that has the same amplitude, positive and negative going. If it isn't adjusted properly, the amp has a higher harmonic distortion. It doesn't sound as clean. Some players like the dirt of an unbalanced amp.
To answer your other question about feeding in a low E signal from a bass. The frequency isn't so much of an issue. It doesn't need to be exactly 40Hz. This is a standard that Ampeg uses to ensure that things are done consistently. You want a low frequency, it can be 100 Hz, for example. It is best if you use a clean signal. A tech will use a signal generator that outputs a pure sine wave with low distortion to do this. If you have a clean input feeding the amp, then you can use what is called a harmonic distortion analyzer on the output to set the balance for minimal harmonic distortion. You don't need to be so fussy when setting the balance though. On the old SVT's you could use a voltmeter between the two test points (K1 and K2) on the back of the amp and adjust the balance to read 0 +/- 0.01 volts. You can still do this on the VR if you didn't want to use the balance LED but you would need to open the amp up to gain access. Instead of a signal generator, you can buy small boxes that run on a 9V battery that will output a sine wave. You can also download tone files from the internet and feed the output from your computer or a music player into your amp.
Can you simply use your bass? The signal from your bass isn't a clean sine wave, it also has harmonics. So it is a complicated waveform. This makes it very hard to set an accurate balance.