There are 3 ways you can buffer your signal. The first is with active pickups or circuitry. Active pickups take a weak signal and amplify them with a preamp and a buffer. Active circuitry works the same way, by boosting frequencies and buffering the signal. If you run active circuitry on your bass, your signal comes out buffered.
Well... I'm going to split hairs here, but only because some of your posts are passing semi-correct ideas off as entirely-correct ones, and I just want to get it straight.
First, saying active pickups use "a preamp and a buffer" is redundant; they only use one gain stage, not two, in the pickups. "If you run active circuitry on your bass, your signal comes out buffered" is mainly true but not always, because some active systems have a passive volume knob at the end, raising the output impedance, which undoes one of the benefits of the buffer. Incidentally, that also is the case with some buffering booster pedals: they take a good buffer and spoil it by adding a passive volume knob.
Some people use pedals with weak buffers (Boss) and stack them to buffer their signal, but this tends to weaken attack response.
Not all Boss buffers are bad--they are not all made the same. I've never heard of anybody intentionally "stacking them" for more buffering.
The best way to buffer with pedals is to use all true bypass pedals and have one, strong, dedicated buffer.
That is ONE good approach, that works for you. But there are multiple ways of implementing "true bypass", and they are not all equally good. So it's a mistake to fetishize true bypass as a basic cure-all, the same way it would be a mistake to assume all buffers are good. It's also not right to start with "a chain of BAD buffers has a negative impact" and extrapolate it to "a chain of GOOD buffers has a negative impact". Additionally, there are plenty of people who have one or more "always on" pedals, and if one of those works well as a buffer while engaged, then there is no need to bother about the type of bypass it has. For that matter, the lineup you described really only makes sense during the parts of a song where you are not using
any of those true-bypass pedals.
I run a 20ft cable to my board, and a 30ft cable from my board, and there is no difference between all that cable (plus what's between my pedals) and a 10ft cable straight in.
Maybe no audible difference with your amp and speakers, and that
is a legitimate measure for your personal case, but that is another story than saying there is no difference. Again, sorry for splitting hairs---most people would never hear the doubled capacitance of the 20 vs the 10, with most amp/cabs.
I did the eyeglass test to see.
I've never heard of any eyeglass test. I googled it, and the ONE result was a post on another forum from somebody named BeZo.
Who is using a dedicated buffer, and what buffer pedal are you using?
I usually have a compressor with a high-quality output "on", acting as a buffer while engaged. The rest of my pedals either have well-designed buffered bypass, or well-designed true bypass. As long as they are built for no audible distortion, and no "pops" while switching, I call it good.